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Fast Results! Homemade Enzyme Cleaner for Cat Urine

  • 12 min read

Pinterest pin on how to remove cat urine smell with a homemade enzyme cleaner

I’ve been dealing with that annoying cat urine smell in my house and I know it can be so frustrating, especially when commercial cleaners don’t seem to work. I recently started trying out a homemade enzyme cleaner for cat urine, and it’s been a game-changer!

There are so many different recipes out there that are natural, budget-friendly, and most importantly, effective. My house finally smells fresh again and those stubborn pet stains are all but gone.   This is how I did it with a great all natural cleaner.

How to Get Rid of Cat Pee Smell

Cat urine smells can be challenging to locate. Usually, it’s just the smell that gives it away. But once you figure out where it is, it’s super important to deal with it quickly. That way, the odor won’t stick around, and it’ll discourage your kitty from going back to that same spot.

Consider making homemade body butter.

Getting Ready to Use Your Homemade Cat Urine Cleaner

When you’re about to use a homemade cleaner, it’s really important to get the area ready first. I usually start by vacuuming up any solid bits and then using paper towels or old rags to blot up any extra liquid.

Make sure to press down instead of rubbing – you don’t want to push the urine further into the carpet. Getting as much of the pet urine out beforehand really helps the cleaner work its magic much better.

After that, double-check that the area is clear of any debris because it can really make it harder for the cleaner to get to the stain and get rid of that smell.

If you’re handling a recent cat pee mishap, taking care of the stain before using your homemade DIY cat urine cleaner will definitely make the cleaning part a lot easier.

Why Use Vinegar-Based DIY Cat Urine Cleaner

I’ve found that a vinegar-based cleaner works really well on things like clothes, bedding, and curtains with cat urine stains.

To whip up this homemade DIY cat urine cleaner, just mix equal parts baking soda and white vinegar.

It’ll bubble up, which actually helps lift the stain! In another container, mix some hydrogen peroxide with a squirt of dish soap, then pour it into a spray bottle for easy use.

When you’re ready to clean up that cat urine stain, just dab it gently with a dry cloth, and then spray your vinegar-based solution on it.

Let it sit for around 15 minutes, and it’ll really help break down that stain. Then, just wipe it off with a damp sponge and rinse the area well with clean water.

Oh, and just a heads-up: don’t use this cleaner on marble or stone surfaces, as the vinegar might damage them.

How to Use Baking Soda and Hydrogen Peroxide Cat Urine Cleaner

If you need an effective homemade DIY cat urine cleaner (works on dog urine as well) to handle both the smell and the stain, a mix of baking soda and hydrogen peroxide might be just the thing.

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Baking soda is famous for getting rid of odors, while hydrogen peroxide works wonders on breaking down organic material in cat pee and other pet accidents.

It’s easy to make this powerful cleaner. Just combine equal parts baking soda and hydrogen peroxide, and then add a bit of dish soap. Transfer the mixture into a spray bottle for convenient application.

Spray it onto the cat urine stain, and let it dry. If the stain is still there, don’t worry – just repeat the process until it’s completely gone.

This combination of baking soda and hydrogen peroxide is a cost-effective and efficient solution for dealing with cat urine stains and odors. Give it a try, and I think you’ll be pleasantly surprised by the results!

To create this powerful cleaner, mix equal parts baking soda and hydrogen peroxide in a container, then add a squirt of dish soap. Pour the mixture into a spray bottle for easy application.

Spray the solution onto the cat urine stain, and let it dry. If additional cleaning is needed, repeat the process until the stain is completely removed.

This baking soda and hydrogen peroxide solution is an effective and budget-friendly way to tackle cat urine stains and odors. Give it a try, and you’ll be amazed at the results!

5 from 2 votes

Homemade Cat Urine Enzyme Cleaner

This solution not only helps in removing the stain but also neutralizes the strong ammonia smell associated with cat urine. However, always remember to test any solution on a small, hidden part of the material or surface before treating the entire stain, especially if you're unsure how it might react.
Prep Time5 minutes
Total Time5 minutes
Yield: 12 ounces


  • 1/2 cup white vinegar
  • 1/2 cup water
  • 2 tablespoons baking soda
  • 1 teaspoon mild dish detergent
  • 1/4 cup hydrogen peroxide 3%


  • Blot the Stain: If the pet urine is fresh, first blot up as much of the pet urine as possible using paper towels or a clean cloth. Press firmly to ensure you soak up as much of the liquid as possible.
  • Prepare the Mixture:
    Start by combining the following ingredients in a large bowl or mixing container:
  • Stir Gently:
    Stir the mixture gently to ensure that all the ingredients are well combined. Be cautious not to create excessive froth.
  • Add Hydrogen Peroxide:
    Once the initial mixture is well-blended, add 1/4 cup of hydrogen peroxide (3%) to the mixture. Hydrogen peroxide helps to break down the odor-causing compounds in cat urine.
  • Stir Again:
    Carefully stir the mixture again to incorporate the hydrogen peroxide.
  • Test on a Small Area:
    Before applying the cleaner to the stained area, it's a good practice to test a small, inconspicuous area of the affected surface to ensure it won't damage or discolor the material.
  • Apply to the Stain:
    Pour the prepared cleaner directly onto the cat urine stain. Ensure that you saturate the area completely.
  • Gently Scrub:
    Using a clean cloth or a soft brush, gently scrub the stained area in a circular motion. This helps the cleaner penetrate the stain and neutralize the odor.
  • Blot and Rinse:
    After scrubbing, blot the treated area with a clean, dry cloth to remove excess moisture and residue. Then, rinse the area thoroughly with clean water to remove any remaining cleaner.
  • Allow to Air Dry:
    Let the treated area air dry naturally. This can take a few hours to a day, depending on the size and absorbency of the surface.
  • Repeat If Necessary:
    For stubborn stains and odors, you may need to repeat the process. Ensure the area is completely dry before determining if further treatment is needed.


This homemade cat urine cleaner is an effective and affordable solution for removing cat urine stains and odors from various surfaces. Remember to follow the instructions carefully and test on a small area first to avoid any potential damage to your belongings.

Citrus Enzyme Cleaner

For a fresh-smelling DIY cat urine cleaner, consider trying a citrus enzyme cleaner made from orange, sugar, and yeast. This type of cleaner is highly effective in breaking down cat urine odor and pet stains while leaving behind a pleasant citrus scent.

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To prepare the citrus enzyme cleaner, simply combine orange scraps, brown sugar, and water in a plastic bottle. Allow the mixture to ferment for about two weeks, during which the orange scent will help to eliminate strong odors.

Once fermented, strain the liquid into another container and dilute it with water before using it to clean cat urine stains.

While there are commercial citrus enzyme cleaner available, such as Bubba’s Rowdy Friends Pet Stain & Odor Terminator, creating your own homemade version is an affordable and eco-friendly alternative.

DIY Citrus Cat Urine Enzyme Cleaner

If you’re looking for a homemade DIY cat urine cleaner that leaves a lovely scent behind, give a citrus enzyme cleaner made from orange, sugar, and yeast a try. It’s really effective at breaking down cat urine stains and smells, plus it leaves a nice citrus aroma.

To make the citrus enzyme cleaner, all you need to do is combine orange scraps, brown sugar, and water in a plastic bottle. Let the mix ferment for about two weeks – the orange scent will help to tackle those strong odors.

After it’s done fermenting, strain the liquid into another container, and dilute it with water when you’re ready to clean up cat urine stains.

Of course, there are commercial citrus enzyme cleaner out there, like Bubba’s Rowdy Friends Pet Stain & Odor Terminator, but making your own homemade version is a more budget-friendly and eco-conscious option.

Apple Cider Vinegar Alternative

If you’re looking for a homemade DIY cat urine cleaner with a sweeter scent than white vinegar, you might want to try using apple cider vinegar instead.

Apple cider vinegar has acid content that’s great at getting rid of those odor-causing bacteria and neutralizing the basic components in cat pee.

Making an apple cider vinegar cleaner is super easy – just mix equal parts water and apple cider vinegar in a bowl. Put the solution on the stained area and let it sit for around 5 minutes. After that, just use paper towels to remove any leftover liquid.

This apple cider vinegar method is a natural and efficient way to clean up cat urine stains while leaving your home smelling pleasantly fresh and sweet.

Preventing Future Cat Urine Accidents

In order to prevent future cat pee incidents, it’s really important to figure out what’s causing them in the first place. It could be an illness, a change in their diet, or something in their environment.

Keeping the litter box clean and making sure your cat has a comfy, stress-free space can also go a long way in preventing accidents.

As I already mentioned, it’s best to tackle any existing pet stains right away and use a pet-safe homemade DIY cat urine cleaner to get ridof the smell. This will keep your kitty from going back to that same spot again.

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And don’t forget to keep their litter box clean and tidy; if it’s too dirty, your cat might look for alternative places to do their business.

By staying on top of these things, you’ll be more likely to avoid cat pee accidents in the future and keep your home fresh and clean for both you and your furry friend!

Dealing with Persistent Cat Pee Smell

Even if you’ve tried some of the homemade DIY cat urine cleaner recipes and are still dealing with lingering smells, don’t give up!

An enzyme cleaner, vinegar, and baking soda can all be really useful in getting rid of cat urine odors. But, you might need to apply the cleaner a few times or try mixing different cleaners to get the results you want.

Or, you could try combining baking soda and vinegar, put it on the stain, and let it sit for a few minutes before wiping it off. Just stay patient and determined, and eventually, the smell will go away.

Keeping your home free of cat urine odors is not only good for you, but also for your family and guests. With the right homemade cleaners and techniques, you can have a fresh-smelling home again.

How do I get pet pee smell out of clothes, mattress or a sofa?

Act quickly. Rinse and blot the affected area with cold water. Pre-treat with an enzyme cleaner designed for pet odors. Wash the clothes in cold water with detergent. Check for lingering smell before drying. Repeat the process if needed. If unsure, consult a professional cleaner.

Another option would be to sprinkle baking soda on it after trying the above solution.  Wait at least an hour or two and then vacuum up.

Can I make my own enzyme cleaner for cat urine?

Yes, you can make your own enzyme cleaner for cat urine. Mix white or apple cider vinegar and water in a 1:1 ratio, put the mixture in a spray bottle, and spray the stain.

Additionally, you can make an enzyme cleaner by combining citrus peels, lukewarm water, brown sugar, and yeast in a plastic bottle, letting it ferment, and then straining out the citrus peels before transferring it to an airtight container.

What household products can you use to clean cat urine?

To clean cat pee, you can use white vinegar, baking soda, an enzymatic cleaner, and water. Start by soaking up the cat pee with a paper towel or cloth. Then spray the area with white vinegar and let it sit for several minutes before wiping with more paper towels.

Follow this by sprinkling baking soda on the stain and scrubbing with a brush, then rinse with water. Finally, spray an enzymatic cleaner onto the area to remove any lingeringp et odors.

How do you neutralize the smell of cat urine?

To effectively neutralize the smell of cat urine, first thoroughly clean the soiled area with a solution of vinegar and water.

Then apply an enzyme treatment to break down and evaporate the uric acid, leaving your home free from unpleasant pet odors.

How do you make homemade floor cleaner for cat urine?

To make your own homemade floor cleaner for cat pee, mix one cup of white vinegar with one cup of warm water in a bowl. Put the mixture into a spray bottle, and spray the area that has been affected by cat pee.

Then wash the floor with soapy water, dabbing the stain with a sponge soaked in a solution of one and a half cups of warm water and half a cup of white vinegar. This will help neutralize the alkaline cat pee and make your floors clean again.

Does vinegar work as an enzyme cleaner?

Based on the given advice, it is clear that vinegar and lemon are not effective as an enzyme cleaner.

However, they can be used as acidic cleaners to dissolve greasy or sticky soils from a surface.

2 thoughts on “Fast Results! Homemade Enzyme Cleaner for Cat Urine”

5 from 2 votes (2 ratings without comment)

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